Imaging and Quantification of Myelin Integrity After Injury With Spectral Confocal Reflectance Microscopy

DG Gonsalvez; SW Yoo; JL Fletcher; RJ Wood; GA Craig; SS Murray; J Xiao

Journal article

Imaging and Quantification of Myelin Integrity After Injury With Spectral Confocal Reflectance Microscopy

DG Gonsalvez, SW Yoo, JL Fletcher, RJ Wood, GA Craig, SS Murray, J Xiao

Frontiers in Molecular Neuroscience | FRONTIERS MEDIA SA | Published : 2019

DOI: 10.3389/fnmol.2019.00275

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Abstract

Developing a high-throughput approach to quantify the extent of myelin integrity in preclinical models of demyelinating diseases will enhance our capacity to identify novel therapies for myelin repair. In light of the technical limitations of electron microscopy and immunohistochemical analyses of myelination, we have utilized a novel imaging technique, spectral confocal reflectance (SCoRe) microscopy. SCoRe takes advantage of the optically reflective properties of compact myelin, allowing the integrity of compact myelin to be quantified over the course of the cuprizone-induced model of central demyelination. We applied SCoRe imaging on fixed frozen brain sections. SCoRe analysis of control ..

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University of Melbourne Researchers

David Gonsalvez Author

Jessica Fletcher Author

Rhiannon Wood Author

Simon Murray Author

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Grants

Awarded by University of Melbourne

Funding Acknowledgements

We would like to thank all grant sponsors: Australian Research Council Discovery Project Grant (#DP180102397) and the Australian National Health and Medical Research Council (NHMRC) Project Grant (#APP1058647) to JX; NHMRC ECF Fellowship (#APP1111041) to DG; Multiple Sclerosis Research Australia Postdoctoral Fellowship (#14-056) to JF; and the Sir John Eccles Vacation Scholarship and the Melbourne Research scholarship to SY and GC. Confocal imaging was performed at the Biological Optical Microscope Platform, The University of Melbourne.